By Gilson Khang, Moon Suk Kim, Hai Bang Lee
Tissue engineering has been well-known as delivering an alternate strategy to whole-organ and tissue transplantation for diseased, failed, or malfunctioned organs. To reconstruct a brand new tissue through tissue engineering, the next triad parts are wanted: (1) cells that are harvested and dissociated from the donor tissue; (2) biomaterials as scaffold substrates within which cells are connected and cultured, leading to implantation on the wanted web site of the functioning tissue; and (3) progress elements which advertise and/or hinder mobilephone adhesion, proliferation, migration, and differentiation. of those 3 key elements, scaffolds play a severe position in tissue engineering. This well timed ebook makes a speciality of the education and characterization of scaffold biomaterials for the appliance of tissue-engineered scaffolds. extra importantly, it serves as an experimental guidebook at the standardization of the fabrication procedure and characterization of scaffolding expertise.
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Extra resources for A Manual for Biomaterials/Scaffold Fabrication Technology
3). • The prepared scaffolds are physically stable and manageable. • Highly porous 3D scaffolds with porosities up to 99% and median pore diameters up to 400 µm have been prepared using the ice-particle leaching method. Fig. 2 Image of sieved ice microparticle. qxd 5/12/2007 10:48 AM Page 29 FA Characterisations 29 Fig. 3 Image of porous 3D scaffolds fabricated by the ice particle– leaching method. Fig. 4 Pore sizes of PLGA scaffolds fabricated by the ice particle– leaching method with different ratios of polymer and ice.
3. 4. 5. 6. 7. 8. 4 Characterisations • Using the demanded sieve, the sizes of the salt particulates are controlled. The shape of the salt is measured through photomicrographs. • The salt particulates are almost square-shaped (Fig. 4). • The salt-leaching method provides high porosity up to 97%, and median pore diameters up to 140 µm are prepared. • The porosity and pore size can be independently controlled by varying the amount and size of the salt particles, respectively. 1). qxd 5/12/2007 10:48 AM Page 17 FA Characterisations 17 (a) (b) Fig.
Qxd 5/17/2007 8:18 PM Page 2 FA 2 Introduction It has been recognised that tissue engineering offers an alternative technique to tissue transplantation for diseased or malfunctioned organs. Millions of patients suffer from end-stage organ failure or tissue loss each year. In the United States alone, at least eight million surgical operations are carried out annually, requiring a total national healthcare cost exceeding US$400 billion per year [1, 2]. In the case of cardiovascular disease, approximately 500 000 coronary artery bypass surgeries are conducted each year in the United States .
A Manual for Biomaterials/Scaffold Fabrication Technology by Gilson Khang, Moon Suk Kim, Hai Bang Lee